2021 年第 1 期 第 16 卷

微小RNA-206调控膜联蛋白A2表达对肺癌细胞增殖与迁移和侵袭能力的影响

The effect of microRNA-206 regulating annexin A2 expression on the proliferation, migration and invasion ability of lung cancer cells

作者：陈晓芸刘欢唐潇潇崔艳伟

英文作者：Chen Xiaoyun Liu Huan Tang Xiaoxiao Cui Yanwei

单位：大连大学附属中山医院呼吸科116001

英文单位：Department of Respiratory Zhongshan Hospital Affiliated to Dalian University Dalian 116001 China

关键词：肺癌细胞；微小RNA-206；膜联蛋白A2；增殖；迁移；侵袭

英文关键词：Lungcancercells;MicroRNA-206;AnnexinA2;Proliferation;Migration;Invasion 

• 摘要：

• 目的 分析微小RNA（miR）-206调控膜联蛋白A2表达对肺癌细胞增殖、迁移和侵袭能力的影响。方法取人肺腺癌A549细胞分为miR-NC组、miR-206组、miR-206 inhibitor组、miR-206 inhibitor NC组，依照分组转染质粒；采用荧光素酶报告实验检测分析miR-206与膜联蛋白A2的关系，采用实时荧光定量聚合酶链反应和蛋白质印迹法检测miR-206、膜联蛋白A2 mRNA和膜联蛋白A2表达水平；并检测各组细胞增殖、迁移和侵袭能力。结果 miR-206组膜联蛋白A2表达水平明显低于miR-NC组［（0.10±0.03）比（0.35±0.08）］，差异有统计学意义（P<0.05）。miR-NC组、miR-206组、miR-206 inhibitor组、miR-206 inhibitor NC组miR-206表达水平差异有统计学意义（P<0.05），其中miR-206组最高，miR-206 inhibitor组最低，但各组膜联蛋白A2 mRNA表达水平差异无统计学意义（P>0.05）；各组膜联蛋白A2表达水平差异有统计学意义（P<0.05），其中miR-206组最低（0.10±0.03），miR-206 inhibitor组最高（0.73±0.12）；各组细胞增殖率、细胞迁移和侵袭活性差异有统计学意义（P<0.05），其中miR-206组最低，miR-206 inhibitor组最高。结论 miR-206可通过靶向调控膜联蛋白A2表达而抑制肺癌细胞的增殖、迁移和侵袭能力，可作为潜在的肺癌治疗新靶点。

• Objective To analyze the effect of microRNA(miR)-206 regulating annexin A2 expression on the proliferation, migration and invasion ability of lung cancer cells. Methods Human lung adenocarcinoma A549 cells were divided into miR-NC group, miR-206 group, miR-206 inhibitor group, and miR-206 inhibitor NC group, and plasmids were transfected according to the grouping; the relationship between miR-206 and annexin A2 was detected and analyzed by luciferase report experiment. Luciferase report test was used to detect the relationship between miR-206 and annexin A2. Real-time fluorescence quantitative polymerase chain reaction and Western blotting were used to detect the expression of miR-206, annexin A2 mRNA and annexin A2, and the ability of cell proliferation, migration and invasion. Results The expression level of annexin A2 in the miR-206 group was significantly lower than that in the miR-NC group［（0.10±0.03） vs （0.35±0.08）］, and the difference was statistically significant(P<0.05). There were significant differences in miR-206 expression levels among miR-NC group, miR-206 group, miR-206 inhibitor group and miR-206 inhibitor NC group（P<0.05）, with the highest in the miR-206 group and the lowest in the miR-206 inhibitor group, but there were no significant differences in the annexins A2 mRNA expression levels among the 4 groups(P>0.05). There were significant differences in the expression of annexin A2 among different groups（P<0.05）, with the lowest in miR-206 group（0.10±0.03） and the highest in miR-206-inhibitor group（0.73±0.12）. There were significant differences in cell proliferation rate, cell migration and invasion activity among the 4 groups(P<0.05), with the lowest in miR-206 group and the highest in miR-206 inhibitor group. Conclusion miR-206 can inhibit the proliferation, migration and invasion of lung cancer cells by targeting regulation of annexin A2 expression, and can be used as a potential new target for the treatment of lung cancer.