2023 年第 11 期 第 18 卷

多瘤病毒增强活化子3和红细胞生成素产生肝细胞受体A2在脑胶质母细胞瘤中的作用

The role of polyomavirus enhancer activator 3 and erythropoietin-producing hepatocellular receptor A2 in glioblastoma of the brain

作者：麦麦提依明·托合提张诚高峰王继超吴永刚

英文作者：Mamatemin Tohti Zhang Cheng Gao Feng Wang Jichao Wu Yonggang

单位：新疆维吾尔自治区人民医院神经外科，乌鲁木齐830001

英文单位：Department of Neurosurgery People′s Hospital of Xinjiang Uygur Autonomous Region Urumqi 830001 China

关键词：胶质母细胞瘤；多瘤病毒增强活化子3；红细胞生成素产生肝细胞受体A2

英文关键词：Glioblastoma；Polyomavirusenhanceractivator3；Erythropoietin-producinghepatocellularreceptorA2

• 摘要：

• 目的  探讨多瘤病毒增强活化子3（PEA3）、红细胞生成素产生肝细胞受体A2（EPHA2）在脑胶质母细胞瘤中的作用。方法  构建人脑星形胶质母细胞瘤U87细胞基因转染模型，将细胞系分为5组：空白组、PEA3干扰组、PEA3干扰空载组、EPHA2干扰组、EPHA2干扰空载组。通过细胞计数试剂盒8(CCK-8)实验检测细胞增殖率，细胞划痕实验检测细胞迁移能力，Transwell实验检测细胞侵袭能力。结果  CCK-8检测细胞增殖结果显示，PEA3干扰组和EPHA2干扰组的细胞增殖率均明显低于空白组［（59.7±1.3）%、（59.5±0.7）%比（67.8±1.3）%］（P＜0.01或P＜0.001），PEA3干扰空载组和EPHA2干扰空载组的细胞增殖率与空白组比较差异均无统计学意义（均P＞0.05）。细胞划痕实验结果显示，PEA3干扰组和EPHA2干扰组的划痕愈合率低于空白组［（31.7±1.1）%、（23.0±2.6）%比（42.8±8.8）%］，差异均有统计学意义（均P＜0.05），PEA3干扰空载组和EPHA2干扰空载组划痕愈合率与空白组比较差异均无统计学意义（均P＞0.05）。Transwell检测细胞侵袭能力结果显示，PEA3干扰组和EPHA2干扰组的侵袭细胞数明显少于空白组［（643±20）、（542±165）个比（1 225±70）个］（均P＜0.001），而空白组与PEA3干扰空载组和EPHA2干扰空载组侵袭细胞数比较差异均无统计学意义（均P＞0.05）。结论  干扰PEA3基因和EPHA2基因能明显降低脑胶质母细胞瘤细胞的增殖、迁移、侵袭能力。

• Objective  To investigate the role of polyomavirus enhancer activator 3(PEA3) and erythropoietin-producing hepatocellular receptor A2(EPHA2) in glioblastoma of the brain. Methods  The gene transfection model of glioblastoma U87 cells was constructed. The cell lines were divided into five groups: blank group, PEA3 interference group, PEA3 interference empty group, EPHA2 interference group and EPHA2 interference empty group. The proliferation rate was detected by cell count kit-8 (CCK-8) test, the migration ability was detected by cell scratch test, and the invasion ability was detected by Transwell test. Results  CCK-8 test showed that compared with the blank group, the cell proliferation rate of PEA3 interference group and EPHA2 interference group decreased significantly［（59.7±1.3）%, （59.5±0.7）% vs （67.8±1.3）%］(P＜0.01 or P＜0.001). There was no significant difference between the blank group and PEA3 interference empty group and EPHA2 interference empty group in cell proliferation rate (both P＞0.05). Cell scratch test showed that compared with the blank group, the cell scratch healing rate of PEA3 interference group and EPHA2 interference group decreased［（31.7±1.1）%, （23.0±2.6）% vs （42.8±8.8）%］(both P＜0.05). There was no significant difference between the blank group and PEA3 interference empty group and EPHA2 interference empty group in scratch healing rate (both P＞0.05). Transwell test Results   showed that compared with the blank group, the number of invasive cells in PEA3 interference group and EPHA2 interference group decreased significantly ［（643±20）,（542±165）cells vs （1 225±70）cells］(both P＜0.001), and there was no significant difference between the blank group and PEA3 interference empty group and EPHA2 interference empty group(both P＞0.05). Conclusion  Interference with PEA3 gene and EPHA2 gene can significantly reduce the proliferation, migration and invasion of glioblastoma of the brain.