2018 年第 12 期 第 13 卷

荷叶总黄酮抑制前列腺癌细胞增殖的研究

Total flavonoids of lotus leaves inhibits human-derived prostate cancer cell proliferation

作者：刘正欢廖邦华夏超成思航张永刚刘嘉铭

英文作者：

单位：610041成都，四川大学华西医院泌尿外科泌尿外科研究所［刘正欢为四川大学华西临床医学院外科学（泌尿外科）在读硕士研究生］

英文单位：

关键词：前列腺癌；黄酮；细胞增殖；细胞凋亡；细胞周期

英文关键词：

• 摘要：

• 【摘要】目的    探讨荷叶总黄酮抑制前列腺癌细胞增殖的作用及机制。方法    提取并分离纯化荷叶总黄酮，分析荷叶总黄酮对体外LNCap细胞及DU145细胞和PC3细胞的细胞增殖活性、相关蛋白表达、细胞周期及细胞凋亡的影响。采用动物实验检测荷叶总黄酮体内抑制前列腺癌细胞增殖作用。结果    荷叶总黄酮对3种前列腺癌细胞增殖均有抑制作用，且存在剂量-效应关系。荷叶总黄酮可下调3种前列腺癌细胞中Ki67蛋白表达，诱导DU145细胞及LNCap细胞的细胞周期停滞在S期，并诱导3种前列腺癌相关细胞凋亡。动物实验中，3种荷瘤裸鼠体质量均降低，且荷叶总黄酮100、200、300 mg/kg灌胃喂养组肿瘤体积生长速率均明显低于纯净水灌胃喂养组（均P＜0.001）。结论    荷叶总黄酮在体外实验及动物实验中均可抑制前列腺癌细胞增殖，可能与其诱导细胞周期停滞及增加细胞凋亡有关。

• 【Abstract】Objective    To observe the inhibitory effect and mechanism of total flavonoids of lotus leaves on human-derived prostate cancer cell proliferation. Methods    Total flavonoids of lotus leaves were extracted and purified. After treatment with total flavonoids of lotus leaves, cell proliferation, protein expression, cell cycle and apoptosis of prostate cancer LNCap, DU145 and PC3 cells in vitro were tested by CCK, western blotting and flow cytometry. Inhibitory effect of total flavonoids on human-derived prostate cancer cells in vivo was tested through animal experiment. Results    Total flavonoids of lotus leaves could inhibit the 3 kinds of prostate cancer cells in a dose-dependent manner. It down-regulated Ki67 protein expression, induced DU145 and LNCap cell cycle arrest at S phase and cell apoptosis. In mice experiment, the tumor growth rate in total flavonoids 100, 200, 300 mg/kg intragastric feeding groups was significantly lower than that in purified water feeding group(P<0.001). Conclusion    Total flavonoids of lotus leaves can inhibit human-derived prostate cancer cell proliferation in vitro and mice by probably causing cell cycle arrest and increasing apoptosis.