2021 年第 2 期 第 16 卷

微小RNA-133a表达水平与胫骨干骨折延迟愈合的相关性

Correlation between microRNA-133a expression level and delayed union of tibia shaft fracture

作者：陈萍1胡学昱2薄舒心1王倩1杨晨1

英文作者：Chen Ping1 Hu Xueyu2 Bo Shuxin1 Wang Qian1 Yang Chen1

单位：1空军军医大学第一附属医院骨科手术室，西安710032；2空军军医大学第一附属医院脊柱外科，西安710032

英文单位：1Orthopedic Operating Room the First Affiliated Hospital of Air Force Medical University Xi′an 710032 China; 2Department of Spinal Surgery the First Affiliated Hospital of Air Force Medical University Xi′an 710032 China

关键词：

英文关键词：Tibiashaftfracture;MicroRNA-133a;Delayedunion;Corebindingfactor-2;Bonemorphogeneticprotein 

• 摘要：

• 目的 分析微小RNA（miR）-133a表达水平与胫骨干骨折延迟愈合的相关性。方法 选取2014年1月至2018年12月空军军医大学第一附属医院骨科收治的35例胫骨干骨折延迟愈合患者为延迟愈合组，选取同期35例胫骨干骨折正常愈合的患者为对照组。比较2组患者年龄、性别等一般资料，比较2组的骨形态发生蛋白（BMP）、核心结合因子（RUNX）2及miR-133a水平，采用多因素Logistic回归方法对骨折延迟愈合的相关因素进行分析，并对BMP、RUNX2水平与miR-133a的相关性进行分析。结果 延迟愈合组患者年龄及吸烟人数比例大于对照组（均P＜0.05）。延迟愈合组患者的miR-133a水平明显高于对照组，而RUNX2及BMP水平明显低于对照组［（1.21±0.23）比（0.85±0.19）、（0.45±0.13）比（0.68±0.19）、（135±33）ng/L比（166±42）ng/L］，差异均有统计学意义（均P＜0.05）。多因素Logistic分析示miR-133a是骨折延迟愈合的危险因素，而RUNX2及BMP则是其保护因素（比值比=536.138、0.000、0.967，均P＜0.05）；且RNUX2及BMP水平均与miR-133a水平呈负相关，即miR-133a越高，RNUX2及BMP越低（r=-0.736、-0.705，均P＜0.001）。结论 胫骨干骨折患者体内miR-133a过度表达抑制RNUX2及BMP表达，从而影响骨折的正常愈合。

• Objective To analyze the correlation between microRNA(miR)-133a expression level and delayed union of tibia shaft fracture. Methods From January 2014 to December 2018, 35 patients with delayed union of tibial shaft fractures admitted to the Department of Orthopedics, the First Affiliated Hospital of Air Force Medical University were selected as the delayed union group, and 35 patients with normal healing of tibia shaft fracture in the same period were selected as the control group. The age, gender and other general information of the two groups were compared. The levels of bone morphogenetic protein (BMP), core binding factor (RUNX) 2 and miR-133a were compared between the two groups. Multivariate Logistic analysis was used to analyze the factors affecting delayed fracture healing, and the correlation between BMP, RUNX2 levels and miR-133a were analyzed.  Results  The age and proportion of smoking in delayed union group were higher than those in control group(both P<0.05). The miR-133a level of delayed union group was significantly higher than those in the control group, and the RUNX2 and BMP levels in delayed union group were significantly lower than those in control group［（1.21±0.23） vs （0.85±0.19）, （0.45±0.13） vs （0.68±0.19）, （135±33）ng/L vs （166±42）ng/L］(all P<0.05). Multivariate Logistic analysis showed that miR-133a was a risk factor for delayed fracture union, while RUNX2 and BMP were protective factors(odds ratio=536.138, 0.000, 0.967; all P<0.05).  RNUX2 and BMP levels were negatively correlated with miR-133a level, the higher miR-133a was, the lower RNUX2 and BMP were (r=-0.736, -0.705; both P<0.001). Conclusion  The Overexpression of mir-133a inhibits the expression of RNUX2 and BMP in patients with tibia shaft fracture, thus affecting the normal union of fracture.