2021 年第 10 期 第 16 卷

联合检测血清长链非编码RNA小核仁RNA宿主基因16和微小RNA-16-5p对胃癌的诊断价值

The diagnostic value of serum long non-coding RNA small nucleolar RNA host gene 16 combined with microRNA-16-5p detection for gastric cancer

作者：杨季1彭波2李军1陈雯雯1李周彤昕1

英文作者：Yang Ji1 Peng Bo2 Li Jun1 Chen Wenwen1 Li Zhoutongxin1

单位：1武汉市第三医院检验科430000；2武汉市第三医院消化内科430000

英文单位：1Department of Clinical Laboratory Wuhan Third Hospital Wuhan 430000 China; 2Department of Gastroenterology Wuhan Third Hospital Wuhan 430000 China

关键词：胃癌；长链非编码RNA小核仁RNA宿主基因16；微小RNA-16-5p

英文关键词：Gastriccancer;Longnon-codingRNAsmallnucleolarRNAhostgene16;MicroRNA-16-5p

• 摘要：

• 目的  探讨血清长链非编码RNA小核仁RNA宿主基因16(lncRNA SNHG16)联合微小RNA（miR）-16-5p检测对胃癌的诊断价值。方法 选取武汉市第三医院2018年1月至2020年1月收治的102例胃癌患者作为胃癌组，另选取同期102名体检健康者为对照组。对比2组血清lncRNA SNHG16、miR-16-5p水平，分析血清lncRNA SNHG16、miR-16-5p水平与胃癌患者临床及病理参数的关系，Pearson相关性方法分析血清lncRNA SNHG16与miR-16-5p水平的相关性，受试者工作特征（ROC）曲线分析血清lncRNA SNHG16、miR-16-5p水平对胃癌的诊断价值。结果 胃癌组血清lncRNA SNHG16水平明显高于对照组，miR-16-5p水平明显低于对照组［（2.03±0.17）比（1.69±0.34）、（0.56±0.06）比（0.82±0.28）］(均P＜0.001)。胃癌患者血清lncRNA SNHG16、miR-16-5p水平与TNM分期、侵袭程度、淋巴结转移、远处转移相关(均P＜0.05)。Pearson相关性分析结果 显示，胃癌患者血清lncRNA SNHG16与miR-16-5p水平呈负相关(r=-0.405，P＜0.001)。ROC曲线分析显示，lncRNA SNHG16与miR-16-5p联合［曲线下面积（AUC）=0.903，95%置信区间0.854～0.940］诊断胃癌的AUC大于lncRNA SNHG16(AUC=0.816，95%置信区间0.755～0.866)、miR-16-5p(AUC=0.826，95%置信区间0.766～0.875)单独检测。结论 胃癌患者血清lncRNA SNHG16水平显著提升，miR-16-5p水平显著降低，二者呈负相关，联合检测血清lncRNA SNHG16和miR-16-5p水平可提升胃癌诊断价值。

• Objective To explore the diagnostic value of serum long non-coding RNA small nucleolar RNA host gene 16(lncRNA SNHG16) combined with microRNA(miR)-16-5p detection for gastric cancer. Methods Totally 102 patients with gastric cancer admitted to Wuhan Third Hospital from January 2018 to January 2020 was selected as gastric cancer group, and 102 healthy persons undergoing physical examination during the same period were selected as the control group. The serum lncRNA SNHG16 and miR-16-5p levels were compared between the two groups, and the relationship between the serum lncRNA SNHG16, miR-16-5p levels and the clinical and pathological parameters of gastric cancer patients were analyzed. The correlation between the serum lncRNA SNHG16 and miR-16-5p levels was analyzed by Pearson correlation. Receiver operating characteristic(ROC) curve were used to analyzed the value of serum lncRNA SNHG16 and miR-16-5p levels in the diagnosis of gastric cancer. Results The level of serum lncRNA SNHG16 in gastric cancer group was significantly higher than that in control group, and the level of miR-16-5p in gastric cancer group was significantly lower than that in control group［（2.03±0.17） vs （1.69±0.34）, （0.56±0.06） vs （0.82±0.28）］(both P＜0.001). The serum levels of lncRNA SNHG16 and miR-16-5p in patients with gastric cancer were correlated with TNM stage, degree of invasion, lymph node metastasis, and distant metastasis(all P＜0.05). Pearson correlation analysis showed that serum lncRNA SNHG16 were negatively correlated with miR-16-5p level in patients with gastric cancer (r=-0.405， P＜0.001). The ROC curve showed that the area under the curve(AUC) of lncRNA SNHG16+miR-16-5p detection［AUC=0.903, 95% confidence interval(CI): 0.854-0.940］ in the diagnosis of gastric cancer was higher than that of lncRNA SNHG16 (AUC=0.816, 95%CI: 0.755-0.866) and miR-16-5p(AUC=0.826, 95%CI: 0.766-0.875) alone. Conclusions  The level of serum lncRNA SNHG16 in patients with gastric cancer significantly increases, and the level of miR-16-5p significantly reduces. The two indexes are negatively correlated. Combined detection of serum lncRNA SNHG16 and miR-16-5p levels can improve the diagnostic value in gastric cancer.