2022 年第 7 期 第 17 卷

微小RNA-432在肺结核患者血清中的表达水平及对该病的诊断价值评估

The expression and diagnostic value of serum microRNA-432 in patients with tuberculosis

作者：孙雯娜龚文平张秀爽王潇风吴雪琼

英文作者：Sun Wenna Gong Wenping Zhang Xiushuang Wang Xiaofeng Wu Xueqiong

单位：中国人民解放军总医院第八医学中心结核病医学部研究所结核病防控重点实验室结核病诊疗新技术北京市重点实验室，北京100091

英文单位：Senior Department of Tuberculosis the Eighth Medical Center of Chinese People′s Liberation Army General Hospital Tuberculosis Prevention and Control Key Laboratory Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment Beijing 100091 China

关键词：肺结核；结核潜伏感染；微小RNA-432；炎症反应；诊断价值

英文关键词：Tuberculosis;Latenttuberculosisinfection;MicroRNA-432;Inflammatoryresponse;Diagnosticvalue 

• 摘要：

• 目的 探讨微小RNA-432(miR-432)在肺结核患者血清中的表达水平及对该病的诊断价值。方法 纳入2021年1—6月中国人民解放军总医院第八医学中心收治的55例活动性肺结核（ATB）患者和45例结核潜伏感染（LTBI）患者分别为ATB组和LTBI组，另选取同期体检健康者50例为对照组。采用荧光定量聚合酶链反应技术检测结核病患者（LTBI+ATB）和健康对照人群血清miR-432表达水平。采用简单线性回归分析方法分析血清miR-432表达水平与炎症因子水平的相关性。通过绘制受试者工作特征曲线分析miR-432对肺结核的诊断价值。结果 结核病患者(LTBI+ATB)的miR-432表达水平明显高于对照组［（0.005 5±0.001 2）比（0.003 0±0.001 6）］，差异有统计学意义（P＜0.001）。ATB组患者的miR-432表达水平明显高于LTBI组［（0.006 2±0.000 9）比（0.004 8±0.001 3）］，差异有统计学意义（P＜0.001）。结核病患者（LTBI+ATB）中，血清miR-432表达水平与肿瘤坏死因子α和白细胞介素1β水平均呈正相关（r=0.629,P＜0.001；r=0.616,P＜0.001）。血清miR-432区分LTBI与健康人群的截断值为0.003 6，曲线下面积（AUC）为0.821，敏感度为84.4%，特异度为72.0%；区分ATB和健康人群的截断值为0.004 7，AUC为0.942，敏感度为94.6%，特异度为84.0%；区分LTBI和ATB患者的截断值为0.005 5，AUC为0.771，敏感度为70.9%，特异度为71.1%。结论 miR-432在肺结核患者血清中高表达，可以作为肺结核诊断的候选生物标志物。

• Objective To explore the expression and diagnostic value of serum microRNA-432(miR-432) in tuberculosis (TB) patients. Methods A total of 55 patients with avtive TB (ATB) and 45 patients with latent TB infection (LBTI) who were admitted to the Eighth Medical Center of Chinese People′s Liberation Army General Hospital from January 2021 to June 2021 were enrolled as the ATB group and the LTBI group, respectively. Another 50 healthy subjects during the same period were selected as the control group. Quantitative polymerase chain reaction was used to determine the serum miR-432 level in TB patients (LTBI+ATB) and healthy controls. Simple linear regression analysis was performed to detect the correlation between levels of serum miR-432 and inflammatory factor. Receiver operating characteristic (ROC) curve was plotted to evaluate the diagnostic accuracy of miR-432 for TB. Results Compared with the healthy control, the level of serum miR-432 was higher in TB patients (LTBI+ATB)［（0.005 5±0.001 2）vs（0.003 0±0.001 6）］(P＜0.001). The level of miR-432 in ATB group was higher than that in LTBI group ［(0.006 2±0.000 9) vs （0.004 8±0.001 3）］(P＜0.001). The expression level of miR-432 in serum of TB patients (LTBI+ATB) was positively correlated with the levels of tumor necrosis factor-α and interleukin-1β(r=0.629,P＜0.001；r=0.616, P＜0.001). Serum miR-432 had the ability to distinguish LTBI patients from healthy control with the area under the curve (AUC) of 0.821 at the cut-off value of 0.003 6, and the sensitivity and specificity were 84.4% and 72.0% respectively. Serum miR-432 had the ability to distinguish ATB patients from healthy control with AUC of 0.942 at the cut-off value of 0.004 7, and the sensitivity and specificity were 94.6% and 84.0% respectively; serum miR-432 had the ability to distinguish ATB from LTBI with the AUC of 0.771 at the cut-off value of 0.005 5, and the sensitivity and specificity were 70.9% and 71.1%. Conclusion miR-432 is highly expressed in the serum of TB patients and serves as a promising diagnostic biomarker for TB.