2023 年第 12 期 第 18 卷

色素上皮衍生因子在早发性卵巢功能不全患者中的表达变化及其临床意义研究

The expression changes and clinical significance of pigment epithelium-derived factor in patients with premature ovarian insufficiency

作者：杨静陈慧易金玲古丽胡马尔·艾尼瓦尔

英文作者：Yang Jing Chen Hui Yi Jinling Gulihumaer Ainiwaer

单位：新疆医科大学第五附属医院妇科，乌鲁木齐830000

英文单位：Department of Gynecology the Fifth Affiliated Hospital of Xinjiang Medical University Urumqi 830000 China

关键词：早发性卵巢功能不全；色素上皮衍生因子；相关性分析

英文关键词：Prematureovarianinsufficiency;Pigmentepithelium-derivedfactor;Correlationanalysis

• 摘要：

• 目的  探讨色素上皮衍生因子(PEDF)在早发性卵巢功能不全（POI）患者中的表达变化及其临床意义。方法  选取2020年1月至2021年12月于新疆医科大学第五附属医院就诊的10例POI患者为观察组。另外选取同期就诊的卵巢功能评估正常和自然绝经且绝经年龄＞40岁的女性各20名，分别作为正常对照组及绝经对照组。采用双抗体夹心酶联免疫吸附试验法检测3组血清PEDF表达水平；比较3组PEDF表达水平差异并分析PEDF与各项性激素指标的关系。结果  观察组、绝经对照组血清PEDF水平明显低于正常对照组［0.48(0.16,2.49)、1.54(0.49,4.10)μg/L比7.32(4.22,9.91)μg/L］，差异均有统计学意义 (均P<0.05)。但观察组与绝经对照组之间差异无统计学意义（P>0.05）。观察组卵泡刺激素（FSH）、黄体生成素（LH）高于正常对照组，孕酮、抗米勒管激素（AMH）低于正常对照组，差异均有统计学意义（均P<0.05）。3组间睾酮水平差异无统计学意义(P＞0.05)。PEDF与FSH、LH呈负相关（r=-0.410，P=0.003；r=-0.391，P=0.005），与AMH呈正相关(r=0.498，P<0.001)，与雌二醇、孕酮、睾酮无相关性（r=0.070，P=0.631；r=0.211，P=0.142；r=-0.066，P=0.651）。结论   POI患者血清PEDF水平低于卵巢功能正常女性，但与自然绝经女性相比无明显差异。PEDF与卵巢功能相关激素指标存在相关性。

• Objective To discuss the expression changes and clinical significance of pigment epithelium-derived factor(PEDF) in patients with premature ovarian insufficiency(POI). Methods Totally 10 POI patients admitted to the Fifth Affiliated Hospital of Xinjiang Medical University from January 2020 to December 2021 were selected as the observation group. In addition, 20 women with normal and natural menopausal ovarian function evaluation and menopausal age>40 years old were selected as the normal control group and menopausal control group, respectively. Double antibody sandwich enzyme-linked immunosorbent assay was used to detect the expression level of serum PEDF in three groups; the differences in PEDF expression levels were compared among three groups and the relationship between PEDF and various sex hormone indicators was analyzed. Results  The serum PEDF levels in the observation group and menopausal control group were significantly lower than those in the normal control group［0.48(0.16,2.49), 1.54(0.49,4.10)μg/L vs 7.32(4.22,9.91)μg/L］(all P<0.05). However, there was no statistically significant difference between the observation group and the menopausal control group (P>0.05). The follicle-stimulating hormone(FSH) and luteinizing hormone(LH) levels in the observation group were higher than those in the normal control group, while progesterone and anti-Müllerian hormone (AMH) levels were lower than those in the normal control group(all P<0.05). There was no statistically significant difference in testosterone levels among the three groups(P>0.05). PEDF was negatively correlated with FSH and LH (r=-0.410, P=0.003; r=-0.391, P=0.005), and positively correlated with AMH(r=0.498, P<0.001), but no correlation with estradiol, progesterone, and testosterone(r=0.070, P=0.631; r=0.211, P=0.142; r=-0.066, P=0.651). Conclusions  The serum PEDF level in POI patients is lower than those in women with normal ovarian function, but there is no significant difference compared to natural menopausal women. PEDF is correlated with ovarian function related hormones.