2025 年第 1 期 第 0 卷

不稳定型心绞痛患者血清长链非编码RNAOIP5-AS1和微小RNA-137水平与心功能及心血管不良事件的相关性分析

Correlation analysis of serum long non-coding RNA OIP5-AS1 and microRNA-137 levels with cardiac function and adverse cardiovascular events in patients with unstable angina pectoris

作者：邹永伟1 胡小恋1 郑玉群2

英文作者：Zou Yongwei1 Hu Xiaolian1 Zheng Yuqun2

单位：1海南省第二人民医院心内科，五指山572299；2海南省第二人民医院超声科，五指山572299

英文单位：1Department of Cardiology Hainan Second People′s Hospital Wuzhishan 572299 China; 2Department of Ultrasound Hainan Second People′s Hospital Wuzhishan 572299 China

关键词：不稳定型心绞痛；  长链非编码RNAOIP5-AS1；  微小RNA-137；  心功能；  心血管不良事件

英文关键词：Unstableanginapectoris;  Longnon-codingRNAOIP5-AS1;  MicroRNA-137;  Cardiacfunction;  Cardiovascularadverseevents

• 摘要：

• 目的  探讨不稳定型心绞痛（UA）患者血清长链非编码RNA（lncRNA）OIP5-AS1和微小RNA-137（miR-137）水平与心功能及心血管不良事件的相关性。方法  选取2022年5月至2023年8月海南省第二人民医院收治的102例UA患者为观察组。另选取本院同期102名体检健康者为对照组。UA患者根据随访6个月心血管不良事件发生情况分为发生组（n=35）和未发生组（n=67）。采用实时荧光定量聚合酶链反应法检测血清lncRNA OIP5-AS1、miR-137水平；利用Pearson法分析UA患者血清lncRNA OIP5-AS1与miR-137的相关性及lncRNA OIP5-AS1、miR-137与心功能指标的相关性；Logistic回归模型分析影响UA患者发生心血管不良事件的潜在因素；受试者工作特征曲线评估血清lncRNA OIP5-AS1、miR-137水平预测UA患者发生心血管不良事件的效能。结果  观察组患者血清miR-137水平、左心室舒张末期内径（LVEDD）均明显高于/大于对照组，血清lncRNA OIP5-AS1、左心室射血分数（LVEF）水平均明显低于对照组（均P＜0.001）。发生组患者血清miR-137水平、LVEDD均明显高于/大于未发生组，血清lncRNA OIP5-AS1、LVEF水平均明显低于未发生组（均P＜0.001）。血清lncRNA OIP5-AS1与miR-137呈负相关（r=-0.555，P＜0.001）。血清lncRNA OIP5-AS1与LVEDD呈负相关（r=-0.441，P＜0.001），与LVEF呈正相关（r=0.479，P＜0.001）；血清miR-137与LVEDD呈正相关（r=0.466，P＜0.001），与LVEF呈负相关（r=-0.472，P＜0.001）。miR-137、LVEDD升高是影响UA患者发生心血管不良事件的风险因素，而lncRNA OIP5-AS1、LVEF水平升高是其保护因素（均P＜0.05）。血清lncRNA OIP5-AS1、miR-137水平单独预测与二者联合预测UA患者发生心血管不良事件的曲线下面积分别为0.845、0.832、0.924，二者联合优于血清lncRNA OIP5-AS1（Z=2.146、P=0.032）、miR-137（Z=2.114、P=0.035）各自单独预测。结论  发生心血管不良事件的UA患者血清lncRNA OIP5-AS1水平降低、miR-137水平升高，血清lncRNA OIP5-AS1、miR-137是影响UA患者发生心血管不良事件的潜在因素，且二者联合在预测UA患者发生心血管不良事件方面展现出更高的效能。

• Objective  To investigate the correlation of serum long non-coding RNA (lncRNA) OIP5-AS1 and microRNA-137 (miR-137) levels with cardiac function and adverse cardiovascular events in patients with unstable angina pectoris (UA). Methods   A total of 102 UA patients admitted to Hainan Second People′s Hospital from May 2022 to August 2023 were selected as the observation group. A total of 102 healthy people in the same period were selected as the control group. UA patients were divided into occurrence group (n=35) and non-occurrence group (n=67) according to the occurrence of adverse cardiovascular events during 6 months of follow-up. The levels of serum lncRNA OIP5-AS1 and miR-137 were detected by real-time quantitative polymerase chain reaction. The Pearson method was used to analyze the correlation between serum lncRNA OIP5-AS1 and miR-137 in UA patients, and the correlation between lncRNA OIP5-AS1, miR-137 and cardiac function indicators. Logistic regression model was used to analyze the potential factors affecting the occurrence of adverse cardiovascular events in UA patients. The receiver operating characteristic curve was used to evaluate the efficacy of serum lncRNA OIP5-AS1 and miR-137 levels in predicting adverse cardiovascular events in UA patients. Results   The levels of serum miR-137 and left ventricular end diastolic diameter (LVEDD) in the observation group were significantly higher than those in the control group, and the levels of serum lncRNA OIP5-AS1 and left ventricular ejection fraction (LVEF) were significantly lower than those in the control group (all P＜0.001). The levels of serum miR-137 and LVEDD in the occurrence group were significantly higher than those in the non-occurrence group, and the serum levels of lncRNA OIP5-AS1 and LVEF were significantly lower than those in the non-occurrence group (all P＜0.001). There was a negative correlation between serum lncRNA OIP5-AS1 and miR-137 (r=-0.555, P＜0.001). Serum lncRNA OIP5-AS1 was negatively correlated with LVEDD (r=-0.441, P＜0.001) and positively correlated with LVEF (r=0.479, P＜0.001). Serum miR-137 was positively correlated with LVEDD (r=0.466, P＜0.001) and negatively correlated with LVEF (r=-0.472, P＜0.001). The increased levels of miR-137 and LVEDD were the risk factors for adverse cardiovascular events in UA patients, while the increased levels of lncRNA OIP5-AS1 and LVEF were the protective factors (all P＜0.05). The areas under the curve of serum lncRNA OIP5-AS1 and miR-137 in predicting cardiovascular events in UA patients were 0.845, 0.832 and 0.924, respectively. The combination of serum lncRNA OIP5-AS1 and miR-137 was better than that of serum lncRNA OIP5-AS1 （Z=2.146, P=0.032） and miR-137 （Z=2.114, P=0.035） alone. Conclusion   The serum levels of lncRNA OIP5-AS1 were decreased and miR-137 were increased in UA patients with adverse cardiovascular events. The serum levels of lncRNA OIP5-AS1 and miR-137 were potential factors affecting the occurrence of adverse cardiovascular events in UA patients. And the combination of the two shows higher efficacy in predicting cardiovascular adverse events in UA patients.