2025 年第 2 期 第 20 卷

醛缩酶A对膀胱癌细胞生物学的影响及机制研究

Effect of aldolase A on the biology of bladder cancer cells and its mechanism

作者：李建伟蔡芳震刘昌锦

英文作者：Li Jianwei Cai Fangzhen Liu Changjin

单位：福建医科大学附属第二医院泌尿外科，泉州360001

英文单位：Department of Urology Second Affiliated Hospital of Fujian Medical University Quanzhou 360001 China

关键词：膀胱癌；醛缩酶A；上皮-间质转化；β-连环蛋白；细胞增殖

英文关键词：Bladdercancer;AldolaseA;Epithelialmesenchymaltransition;β-catenin;Cellproliferation

• 摘要：

• 目的 探究醛缩酶A对膀胱癌细胞增殖、迁移、侵袭及上皮-间质转化（EMT）的影响及机制。方法 通过慢病毒感染构建干扰或过表达醛缩酶A质粒并转染至T24人膀胱移行细胞癌（T24）细胞中，将细胞分为对照组、空载组、醛缩酶A干扰组和醛缩酶A过表达组。检测醛缩酶A在膀胱癌组织和癌旁组织中的表达。检测细胞增殖、迁移和侵袭情况，同时检测EMT及Wnt/β-连环蛋白信号通路相关蛋白的表达。结果 膀胱癌组织中醛缩酶A的免疫组织化学染色评分及相对表达量均高于癌旁组织［（33±7）分比（17±5）分、（63±6）%比（22±4）%］（均P＜0.001）。细胞计数盒8试验处理24、36和48 h后，醛缩酶A干扰组T24细胞吸光度值均低于空载组，而醛缩酶A过表达组T24细胞吸光度值均高于空载组，差异均有统计学意义（均P<0.05）。醛缩酶A干扰组划痕愈合率低于空载组，醛缩酶A过表达组划痕愈合率高于空载组（均P＜0.05）。醛缩酶A干扰组侵袭细胞数少于空载组，醛缩酶A过表达组侵袭细胞数多于空载组（均P＜0.05）。醛缩酶A干扰组E-钙黏蛋白相对表达量高于空载组，神经钙黏蛋白、波形蛋白、β-连环蛋白、细胞周期素D1、c-Myc基因蛋白相对表达量均低于空载组（均P＜0.05）。醛缩酶A过表达组E-钙黏蛋白相对表达量低于空载组，神经钙黏蛋白、波形蛋白、β-连环蛋白、细胞周期素D1、c-Myc基因蛋白相对表达量均高于空载组（均P＜0.05）。结论 醛缩酶A可促进膀胱癌细胞增殖、迁移、侵袭和EMT，这可能是通过Wnt/β-连环蛋白信号通路发挥影响的。

• Objective To investigate the effect and mechanism of aldolase A on the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of bladder cancer cells. Methods The interference or overexpression plasmids of aldolase A were constructed by lentivirus infection and transfected into T24 human bladder transitional cell carcinoma (T24) cells. The cells were divided into control group, empty vector group, aldolase A interference group and aldolase A overexpression group. The expression of aldolase A in bladder cancer tissues and adjacent tissues was detected. The cell proliferation, migration and invasion were detected, and the expressions of EMT and Wnt/β- catenin signaling pathway related proteins were detected. Results The immunohistochemical staining score and relative expression of aldolase A in bladder cancer tissues were higher than those in adjacent tissues ［(33±7) vs (17±5), (63±6)% vs (22±4)%］（both P＜0.001）. After treatment with Cell Counting Kit-8 assay for 24, 36 and 48 h, the absorbance values of T24 cells in aldolase A interference group were lower than those in the empty vector group, while the absorbance values of T24 cells in aldolase A overexpression group were higher than those in the empty vector group (all P<0.05). The scratch healing rate of aldolase A interference group was lower than that of empty vector group, and the scratch healing rate of aldolase A overexpression group was higher than that of empty vector group (both P<0.05). The number of invasive cells in the aldolase A interference group was less than that in the empty vector group, and the number of invasive cells in the aldolase A overexpression group was more than that in the empty vector group (both P<0.05). The relative expression of E- cadherin in the aldolase A interference group was higher than that in the empty vector group, and the relative expression of N-cadherin, vimentin, β-catenin, cyclin D1, and c-Myc gene protein were lower than those in the empty vector group (all P<0.05). The relative expression of E- cadherin in the aldolase A overexpression group was lower than that in the empty vector group, and the relative expression of N-cadherin, vimentin, β-catenin, cyclin D1, and c-Myc gene protein were higher than that in the empty vector group (all P<0.05). Conclusion Aldolase A can promote the proliferation, migration, invasion and EMT of bladder cancer cells, which may be through the Wnt/β-catenin signaling pathway.