2025 年第 9 期 第 20 卷

淋巴细胞亚群在川崎病患儿体内水平变化及临床意义

Changes and clinical significance of lymphocyte subsets in vivo in children with Kawasaki disease

作者：吴硕1王慧超2李田华3

英文作者：Wu Shuo1 Wang Huichao2 Li Tianhua3

单位：1山东第二医科大学临床医学院，潍坊261053；2中国人民解放军联勤保障部队第九七〇医院威海院区儿科，威海264299；3山东第二医科大学第一附属医院（山东省潍坊市人民医院）小儿内三科，潍坊261000

英文单位：1School of Clinical Medicine Shandong Second Medical University Weifang 261053 China; 2Department of Pediatrics the 970th Hospital of the Joint Service Support Force of the Chinese People′s Liberation Army Weihai Branch Weihai 264299 China; 3the Third Department of Pediatric Medicine the First Affiliated Hospital of Shandong Second Medical University (Weifang People′s Hospital Shandong Province) Weifang 261000 China

关键词：川崎病；淋巴细胞亚群；免疫失衡

英文关键词：Kawasakidisease;Lymphocytesubsets;Immuneimbalance

• 摘要：

• 目的 探讨外周血淋巴细胞亚群在川崎病（KD）患儿体内水平变化及临床意义。方法 选取2021年10月至2022年12月山东省潍坊市人民医院小儿内科收治的99例KD患儿纳入观察组，另选取同期64例以发热为主要表现的普通呼吸道感染患儿纳入对照组。通过流式细胞法检测并比较淋巴细胞亚群指标。采用受试者工作特征曲线评估不同淋巴细胞亚群诊断效能。结果 观察组CD+4、CD+4/CD+8比值、B细胞水平均高于对照组，CD+3、CD+8、自然杀伤细胞水平均低于对照组，差异均有统计学意义(均P＜0.05)。观察组急性期患儿CD+4、CD+4/CD+8比值、B细胞水平均高于非急性期，CD+3、CD+8、自然杀伤细胞水平均低于非急性期患儿，差异均有统计学意义(均P＜0.05)。CD+3、CD+4、CD+8、CD+4/CD+8比值、B细胞、自然杀伤细胞诊断KD急性期患儿的曲线下面积分别为0.494、0.776、0.277、0.924、0.731、0.451，CD+4/CD+8比值的诊断效能最高，其次为CD+4。结论 KD患儿T淋巴细胞亚群指标水平均存在异常，可作为鉴别KD的辅助依据，也可作为评估KD发展和恢复程度的参考指标。

• Objective To investigate the changes and clinical significance of peripheral blood lymphocyte subsets in vivo in children with Kawasaki disease (KD). Methods A total of 99 children with KD admitted to the Department of Pediatric Medicine, Weifang People′s Hospital, Shandong Province from October 2021 to December 2022 were selected as the observation group, and 64 children with common respiratory tract infection with fever as the main manifestation during the same period were selected as the control group. The lymphocyte subsets were detected and compared by flow cytometry. Receiver operating characteristic curve was used to evaluate the diagnostic efficacy of different lymphocyte subsets. Results The levels of CD+4, CD+4/CD+8 ratio and B cells in the observation group were higher than those in the control group, and the levels of CD+3, CD+8 and natural killer cells were lower than those in the control group (all P＜0.05). In the observation group, the levels of CD+4, CD+4/CD+8 ratio and B cells in the acute stage were lower than those in the non-acute stage, and the levels of CD+3, CD+8 and natural killer cells were higher than those in the non-acute stage (all P＜0.05). The area under the curve of CD+3, CD+4, CD+8, CD+4/CD+8 ratio, B cells, and natural killer cells in the diagnosis of acute KD were 0.494, 0.776, 0.277, 0.924, 0.731, and 0.451, respectively. CD+4/CD+8 ratio had the highest diagnostic efficiency, followed by CD+4. Conclusion The levels of T lymphocyte subsets in children with KD are abnormal, which can be used as an auxiliary basis for the identification of KD, and can also be used as a reference index to evaluate the development and recovery of KD.