2026 年第 7 期 第 21 卷

血清微小RNA-214-3p和长链非编码RNA MT1JP表达与宫颈癌患者临床病理特征及预后的关系

Relationship between serum microRNA-214-3p and long non-coding RNA MT1JP expressions and clinicopathological characteristics as well as prognosis in patients with cervical cancer

作者：庞雅君1洪龙年2梁彩霞1陈满瑜1徐祖敏1

英文作者：Pang Yajun1 Hong Longnian2 Liang Caixia1 Chen Manyu1 Xu Zumin1

单位：1广东医科大学附属医院妇科肿瘤及泌尿生殖肿瘤专科，湛江524001；2广东医科大学附属医院妇科，湛江524001

英文单位：1Department of Gynecologic Oncology and Urogenital Oncology Affiliated Hospital of Guangdong Medical University Zhanjiang 524001 China; 2Department of Gynecology Affiliated Hospital of Guangdong Medical University Zhanjiang 524001 China

关键词：宫颈癌；微小RNA-214-3p；长链非编码RNAMT1JP；临床病理特征；预后

英文关键词：Cervicalcancer;MicroRNA-214-3p;Longnon-codingRNAMT1JP;Clinicopathologicalfeatures;Prognosis

• 摘要：

• 目的 探究微小RNA-214-3p（miR-214-3p）、长链非编码RNA MT1JP（lncRNA MT1JP）与宫颈癌患者临床病理特征及预后的关系。方法 回顾性选取2018年6月至2022年6月广东医科大学附属医院收治的116例宫颈癌患者（宫颈癌组）和100例健康体检者（对照组），比较2组miR-214-3p和lncRNA MT1JP水平，分析二者与临床病理特征的关系。根据3年随访生存情况将患者分为死亡组（n=34）和生存组（n=82），比较2组临床资料及miR-214-3p、lncRNA MT1JP。采用多因素Cox回归分析miR-214-3p、lncRNA MT1JP与患者死亡风险的关系，运用限制性立方样条（RCS）及阈值效应分析其剂量-反应关系。采用Pearson相关分析两指标的相关性。分析两指标不同水平与患者预后的关系，绘制Kaplan-Meier生存曲线比较生存率。结果 宫颈癌组血清miR-214-3p高于对照组，lncRNA MT1JP低于对照组（均P＜0.05）。miR-214-3p和lncRNA MT1JP表达水平与肿瘤浸润深度、淋巴结转移、国际妇产科联盟（FIGO）分期、病理分级、血管侵袭及肿瘤分化程度相关（均P＜0.05）。死亡组浸润深度＞1/2肌层、有淋巴结转移、FIGO Ⅲ～Ⅳ期、病理分级G3、低分化比例以及miR-214-3p水平均高于生存组，lncRNA MT1JP水平低于生存组（均P＜0.05）。校正混杂因素后，miR-214-3p和lncRNA MT1JP与死亡风险独立相关（均P＜0.001）。miR-214-3p与lncRNA MT1JP呈负相关性（r=-0.426，P＜0.05）。RCS分析显示二者均与预后呈非线性关系（均P＜0.001）。随访结束时患者总生存率为70.69%，miR-214-3p≥1.23者3年生存率低于＜1.23者生存率；lncRNA MT1JP≤1.58者3年生存率低于＞1.58患者生存率（均P<0.05）。结论 宫颈癌患者血清中miR-214-3p高表达、lncRNA MT1JP低表达，二者与多种侵袭转移相关临床病理特征密切相关，且可作为评估宫颈癌发生及预后的潜在指标。

• Objective To explore the correlations of microRNA-214-3p (miR-214-3p)and long non-coding RNA MT1JP (lncRNA MT1JP) with clinicopathological features and prognosis in patients with cervical cancer. Methods A total of 116 patients with cervical cancer (cervical cancer group) and 100 healthy physical examinees (control group) admitted to Affiliated Hospital of Guangdong Medical University from June 2018 to June 2022 were retrospectively enrolled. The serum levels of miR-214-3p and lncRNA MT1JP were compared between the two groups, and their associations with clinicopathological parameters were analyzed. According to 3-year follow-up outcomes, enrolled patients were divided into death group (34 cases) and survival group (82 cases). Clinical data as well as miR-214-3p and lncRNA MT1JP levels were compared between subgroups. Multivariate Cox regression was performed to analyze the independent correlation of the two indicators with all-cause mortality risk. Restricted cubic spline (RCS) combined with threshold effect analysis was used to assess dose-response relationships. Pearson correlation test was adopted to evaluate the correlation between miR-214-3p and lncRNA MT1JP. Kaplan-Meier survival curves were plotted to compare survival rates stratified by different expression levels of the two biomarkers. Results Serum miR-214-3p was significantly upregulated while lncRNA MT1JP was downregulated in cervical cancer group relative to control group (both P＜0.05). The expression levels of miR-214-3p and lncRNA MT1JP were significantly correlated with depth of tumor invasion, lymph node metastasis, International Federation of Gynecology and Obstetrics (FIGO) stage, pathological grade, vascular invasion and tumor differentiation (all P＜0.05). Compared with survival group, death group presented higher proportion of patients with invasive depth over half of myometrium, lymph node metastasis, FIGO stage Ⅲ-Ⅳ, pathological grade G3 and poor differentiation, accompanied by elevated miR-214-3p and reduced lncRNA MT1JP (all P＜0.05). After adjusting for confounding factors, miR-214-3p and lncRNA MT1JP were independently correlated with mortality risk (both P＜0.001). miR-214-3p was negatively correlated with lncRNA MT1JP (r=-0.426, P＜0.05). RCS analysis revealed a non-linear association between the two indicators and patient prognosis (both P＜0.001). The overall 3-year survival rate was 70.69%. Patients with miR-214-3p ≥1.23 had poorer 3-year survival than those with miR-214-3p＜1.23; patients with lncRNA MT1JP ≤1.58 showed lower survival versus those with lncRNA MT1JP＞1.58 (both P＜0.05). Conclusion Serum miR-214-3p is overexpressed and lncRNA MT1JP is underexpressed in cervical cancer patients. Both molecules are closely linked to multiple clinicopathological features related to tumor invasion and metastasis, and can serve as potential biomarkers for disease onset prediction and prognostic evaluation of cervical cancer.