2019 年第 8 期 第 14 卷

微小RNA-126在结肠癌组织中的表达及临床意义

Expression and clinical significance of microRNA-126 in colon carcinoma

作者：魏海峰米旭光倪志强刘磊李首庆孙牧男马寅芙方艳秋谭岩

英文作者：

单位：130021长春，吉林省人民医院医学诊治实验中心

英文单位：

关键词：结肠癌；微小RNA-126；侵袭和转移

英文关键词：

• 摘要：

• 【摘要】目的    观察微小RNA-126（miR-126）在结肠癌组织中的表达情况，并分析miR-126的表达与结肠癌患者临床病理特征之间的关系。方法    收集2015年1月至2018年9月吉林省人民医院行结肠癌根治术或肠镜下结肠息肉切除术102例患者的手术标本，包括结肠癌70例、腺瘤性息肉32例。采用实时荧光定量聚合酶链反应检测腺瘤组织、结肠癌及其癌旁组织中miR-126的表达。以结肠癌组织miR-126平均表达值为界，将结肠癌病例分为高表达组和低表达组，分析miR-126表达与患者临床特征及病理参数的关系。结果    miR-126在腺瘤型息肉组织中的表达（0.75±0.09）明显低于癌旁组织（1.00），而在高、中、低分化结肠癌组织中的表达［（0.47±0.04）、（0.36±0.12）、（0.29±0.07）］明显低于腺瘤型息肉组织(P＜0.05或P＜0.01)。以miR-126平均表达值（0.37±0.03）为界将结肠癌患者分为高表达组（19例）和低表达组（51例），miR-126的低表达与肿瘤分化程度、TNM分期、是否发生淋巴结转移及远处转移具有明显相关性具有明显相关性（P＜0.05或P＜0.01）。结论    miR-126在结肠癌中低表达，其可能作为抑癌基因与结肠癌的发生和进展有关。

• 【Abstract】Objective    To analyze the expression of microRNA-126（miR-126） and its relation with clinicopathological characteristics of colon carcinoma. Methods    Resection specimens of 70 cases of colon carcinoma and 32 cases of adenomatous colon polyps were collected from January 2015 to September 2018 in Jilin Province People′s Hospital. Expression of miR-126 in adenoma, carcinoma and adjacent tissue were tested by real-time fluorescence quantitative polymerase chain reaction. Patients with colon carcinoma were divided into miR-126 high expression group and low expression group according to the mean expression value. Relation between miR-126 expression and clinicopathological parameters of colon carcinoma was analyzed. Results    Expression of miR-126 in colon adenoma（0.75±0.09） was significantly lower than that in paracancerous tissue（1.00）; the expression in high, medium and poor differentiated colon carcinoma［（0.47±0.04）,（0.36±0.12）,（0.29±0.07）］ was significantly lower than that in adenoma(P＜0.05 or P＜0.01). The mean value of miR-126 expression was （0.37±0.03） and the patients were divided into high expression group (19 cases) and low expression group (51 cases); tumor differentiation degree, TNM stage, lymphatic metastasis and distant metastasis showed significant differences between high expression group and low expression group (P＜0.05 or P＜0.01). Conclusion    MiRNA-126 is low-expressed in colon carcinoma; it may play an important role in the occurrence and progression of colon carcinoma as an anti-oncogene.