2022 年第 1 期 第 17 卷

阿司匹林相关小肠黏膜损伤大鼠模型的构建

Construction of aspirin-related small intestinal mucosal injury model in rats 

作者：阚安琪孙亚梅张杰

英文作者：Kan Anqi Sun Yamei Zhang Jie

单位：首都医科大学附属北京安贞医院消化内科，北京100029

英文单位：Department of Gastroenterology Beijing Anzhen Hospital Capital Medical University Beijing 100029 China

关键词：阿司匹林；小肠黏膜损伤；肠损伤模型

英文关键词：

• 摘要：

• 目的 探讨不同剂量阿司匹林诱导大鼠小肠黏膜损伤动物模型的效果。方法 将30只健康雄性SD大鼠依据随机数字表法分为对照组和模型1组、模型2组、模型3组、模型4组，各6只。对照组大鼠给予0.9%氯化钠注射液2 ml灌胃，模型1组、模型2组、模型3组、模型4组大鼠分别给予50、100、200、300 mg/kg阿司匹林灌胃。连续灌胃14 d。比较5组大鼠体质量增加值、小肠黏膜大体形态变化、病理学评分，空肠和回肠的绒毛高度、隐窝深度及绒毛高度/隐窝深度比值。结果 对照组大鼠活动和进食均正常，各模型组大鼠活动和进食均减少。模型3组和模型4组大鼠体质量增加值均低于对照组［（84±13）、（78±14）g比（123±6）g］（均P＜0.001）。模型4组大鼠的小肠黏膜可见糜烂，绒毛出现大量脱落。模型1组、模型2组、模型3组和模型4组大鼠的空肠、回肠病理学评分均高于对照组，空肠绒毛高度均低于对照组（均P＜0.05）。模型3组和模型4组空肠隐窝深度、空肠绒毛高度/隐窝深度比值和回肠绒毛高度均低于对照组（均P＜0.05）。各组大鼠回肠隐窝深度和回肠绒毛高度/隐窝深度比值比较差异均无统计学意义（均P＞0.05）。结论  采用300 mg/kg阿司匹林连续灌胃14 d可构建阿司匹林相关小肠损伤大鼠模型，该模型主要以小肠绒毛脱落、高度变短为主。

• Objective   To investigate the effect of different doses of aspirin inducing small intestinal mucosal injury model in rats. Methods  Thirty healthy male SD rats were randomly divided into control group, model group 1, model group 2, model group 3 and model group 4, with 6 rats in each group. The rats in the control group were given 0.9% sodium chloride injection by gavage, and the rats in model group 1, model group 2, model group 3 and model group 4 were given 50, 100, 200 and 300 mg/kg aspirin by gavage for 14 d, respectively. The body mass gain, morphological changes of small intestinal mucosa, pathological score, villus height of jejunum and ileum, depth of recess and villus height of jejunum and ileum to depth of recess ratio were compared among the groups. Results  The activity and eating of rats in the control group were normal, and those in each model group were reduced. The increase of body mass in model group 3 and model group 4 were lower than that in the control group ［(84±13), (78±14)g vs (123±6)g］(both P<0.001). The small intestinal mucosa of rats showed erosion and a large number of villus fell off in model group 4. The pathological scores of jejunum and ileum in model group 1, model group 2, model group 3 and model group 4 were higher than those in the control group, and the villus height of jejunum was lower than that in the control group (all P<0.05). The jejunal recess depth, jejunal villus height to recess depth ratio and ileum villus height in model group 3 and model group 4 were lower than those in the control group (all P<0.05). There was no significant difference in ileum recess depth and ileum villus height to recess depth ratio among each group (all P>0.05). Conclusion  The aspirin-related small intestinal mucosal injury rat model can be induced by intragastric administration of 300 mg/kg aspirin for 14 d, which is mainly characterized by villus falling off and becoming shorter in height in the small intestine.